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	    <div id="site-name"><a id="logolink" href="http://www.ncbi.nlm.nih.gov/" title="NCBI Home Page"><img src="primertool.cgi_files/helix.gif" alt="NCBI Logo" title="Link to NCBI Home Page"></a>Primer-BLAST <span class="desc"> Primer-Blast results</span>
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            <a href="http://www.ncbi.nlm.nih.gov/">NCBI</a>/            
            <a href="http://www.ncbi.nlm.nih.gov/tools/primer-blast">Primer-BLAST</a>
            : results:
          Job id=JSID_01_97283_130.14.18.128_9001
            <a href="http://www.ncbi.nlm.nih.gov/tools/primer-blast/primerinfo.html" target="helpWin" class="help">more...</a>
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                    <dl>
                    <dt>Input PCR template</dt> <dd><a href="http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&amp;id=152983466" target="new_entrez">NC_009656.1</a>
Pseudomonas aeruginosa PA7, complete genome&nbsp;</dd>
                    <dt>Range</dt><dd>1776910 - 1778820</dd>
                    <dt>Specificity of primers</dt><dd>Primer pairs are 
specific to input template as no other targets were found in selected 
database: Reference chromosomes (Organism limited to Pseudomonas 
aeruginosa PA7)</dd>     
                    <dt>Other reports</dt>  
                    <dd><a href="#" title="Show statistics" class="resArrowLinkB hiding" id="showStat">Search Summary</a></dd>
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                <table id="params" class="searchInfo"> 
                <caption>Search parameters and other details</caption> 
                <tbody><tr><th><div>Search parameter name</div></th><th><div>Search parameter value</div></th></tr> 
                <tr><td>Number of Blast hits analyzed</td><td>499984</td></tr><tr><td>Entrez query</td><td></td></tr><tr><td>Min total mismatches</td><td>2</td></tr><tr><td>Min 3' end mismatches</td><td>2</td></tr><tr><td>Defined 3' end region length</td><td>5</td></tr><tr><td>Mismatch threshold to ignore targets</td><td>6</td></tr><tr><td>Misprimed product size deviation</td><td>4000</td></tr><tr><td>Max number of Blast target sequences</td><td>50000</td></tr><tr><td>Blast E value</td><td>30000</td></tr><tr><td>Blast word size</td><td>7</td></tr><tr><td>Max candidate primer pairs</td><td>50</td></tr><tr><td>Min PCR product size</td><td>70</td></tr><tr><td>Max PCR product size</td><td>1000</td></tr><tr><td>Min Primer size</td><td>15</td></tr><tr><td>Opt Primer size</td><td>20</td></tr><tr><td>Max Primer size</td><td>25</td></tr><tr><td>Min Tm</td><td>57</td></tr><tr><td>Opt Tm</td><td>60</td></tr><tr><td>Max Tm</td><td>63</td></tr><tr><td>Max Tm difference</td><td>3</td></tr><tr><td>Repeat filter</td><td>AUTO</td></tr><tr><td>Low complexity filter</td><td>Yes</td></tr>  
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            <a id="showGraphic" class="arrowlink resArr showing" href="#">Summary of primer pairs</a>
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<td align="LEFT" valign="CENTER"><a href="#0"><img src="primertool.cgi_files/leftprimer.gif" border="0" height="10" width="7"></a></td>
<td align="LEFT" valign="CENTER"><a href="#0"><img src="primertool.cgi_files/grey.gif" border="0" height="3" width="109"></a></td>
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</div>                        
            <div class="">
            <a href="#" class="arrowlink shown showing resArr" id="showAlign">Detailed primer reports</a>
            <div id="alignInfo" toggle="showAlign" class="resBox shown" name="setToggle">
                <div id="alignments" class="blRes">
                <div class="prPairInfo">
<a name="0"></a><h2>Primer pair 1</h2>
<table>
	<tbody><tr><th></th><th>Sequence (5'-&gt;3')</th><th>Template strand</th><th>Length</th><th>Start</th><th>Stop</th><th>Tm</th><th>GC%</th><th>Self complementarity</th><th>Self 3' complementarity</th></tr>
	<tr><th>Forward primer</th><td>TCGGTGAGGCCGAAGGAGGG</td><td>Plus</td><td>20</td><td>1777151</td><td>1777170</td><td>59.97</td><td>70.00</td><td>4.00</td><td>0.00</td></tr>
	<tr><th>Reverse primer</th><td>GTACCAGCCGGGGCACAACC</td><td>Minus</td><td>20</td><td>1777543</td><td>1777524</td><td>59.97</td><td>70.00</td><td>4.00</td><td>0.00</td></tr>
	<tr class="hidden "><th>Internal oligo</th><td></td><td>Plus</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr>	
	<tr><th>Product length</th><td colspan="9">393</td></tr>
        <tr class="hidden "><th>Product Tm</th><td colspan="9"></td></tr>
        <tr class="hidden "><th>Product Tm - min(OLIGO Tm)</th><td colspan="9"></td></tr>  
        <tr class="hidden "><th>Exon junction</th><td colspan="9"></td></tr>
        <tr class="hidden "><th>Total intron size</th><td colspan="9"></td></tr>       
</tbody></table>
<div class="hidden shown">
    <div class="prPairTl">Products on intended target</div>
    <hr>
    <div class="prPairDtl">
&gt;<a href="http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&amp;id=152983466" target="new_entrez">NC_009656.1</a> Pseudomonas aeruginosa PA7, complete genome &gt;gi|150958624|gb|CP000744.1| Pseudomonas aeruginosa PA7, complete genome
<pre>
product length = 393
Features associated with this product:
   <a href="http://www.ncbi.nlm.nih.gov/nucleotide/152983466?from=1776910&amp;to=1778820&amp;report=gbwithparts" target="new_entrez">nitrous-oxide reductase</a>

Forward primer  1        TCGGTGAGGCCGAAGGAGGG  20
Template        1777151  ....................  1777170

Reverse primer  1        GTACCAGCCGGGGCACAACC  20
Template        1777543  ....................  1777524

</pre>
</div>
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<div class="hidden ">
    <div class="prPairTl">Products on allowed transcript variants</div>
    <hr>
    <div class="prPairDtl"></div>
</div>

<div class="hidden ">
    <div class="prPairTl">Products on potentially unintended templates</div>
    <hr>
    <div class="prPairDtl"></div>
</div>

<div class="hidden ">
    <div class="prPairTl">Products on target templates</div>
    <hr>
    <div class="prPairDtl"></div>
</div>
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